Fig. 6: Inhalation of a TrkB agonist as a promising depression and anxiety treatment in CRS mice.

a Schematic diagram showing the experimental procedures related to 7, 8-DHF inhalation by CRS mice. b Representative immunofluorescence images showing 5-HT and c-Fos colocalization changes in the DRN after the inhalation treatment. Scale bar, 100 µm. Bar chart showing the statistical analysis of 5-HT and c-Fos colocalization in the DRN. Two-tailed t test: t = 4.256, df = 8 ^** P = 0.0028. n = 5 mice. c Changes in 5-HT levels in the brain homogenate supernatants after 7, 8-DHF inhalation treatment. One-way ANOVA: F_{(2, 12)} = 18.07 ^*** P = 0.00024. n = 5 mice. d Representative locomotion tracks and heatmaps in OFT evaluating the antidepressant effects of the 7, 8-DHF inhalation treatment in CRS mice. e Representative activity tracks and heatmaps in the EPM for mice of each group. f Statistical analysis of the total distance, the distance traveled in the center field, and the time spent in the center field of the OFT for mice of each group. Total distance, one-way ANOVA: F_{(2, 27)} = 1.307 ns P = 0.29. Center distance, one-way ANOVA: F_{(2, 27)} = 8.054 ^** P = 0.0018. Center time, Kruskal-Wallis test: ^** P = 0.0066. n = 10 mice. g Statistical analysis of the time spent in and entry numbers to open arms in the EPM for mice of each group. Open arms time, one-way ANOVA: F_{(2, 27)} = 7.380 ^** P = 0.0028. Open arms entry numbers, one-way ANOVA: F_{(2, 27)} = 13.97 ^**** P = 0.000068. n = 10 mice. h Representative tracking of activity in the TST and statistical analysis of the duration of immobility in the TST for mice of each group. One-way ANOVA: F_{(2, 27)} = 5.581 ^** P = 0.0094. n = 10 mice. i Representative schematic diagram of the FST and statistical analysis of the duration of immobility in the FST for mice of each group. Kruskal-Wallis test: ^** P = 0.0040. n = 12 mice. Illustrations created with BioRender.com. Source data are provided as a Source Data file.