Fig. 3: Involvement of the PMS in the extensive PM expansion associated with ring cell inflation. | Nature Communications

Fig. 3: Involvement of the PMS in the extensive PM expansion associated with ring cell inflation.

From: A palisade-shaped membrane reservoir is required for rapid ring cell inflation in Drechslerella dactyloides

Fig. 3

The blue and yellow borders denote the transverse and longitudinal sections, respectively. Magnified views of the areas denoted by the white dotted box in (a–c) are shown at the bottom. a The wavy and curved PM (red arrow) and the ruptured outer cell wall (black arrow) of a partially inflated CR. Scale bars (upper panel = 1 µm; lower panel = 0.5 µm). b, c The PM (red arrow) and the ruptured outer cell wall (black arrow) of inflated CRs. V, vacuole. Scale bars (upper panel = 2 µm; lower panel = 0.5 µm). The images shown in (a–c) were chosen among those collected from at least 5 different CRs. d Percentages of inflated CRs after exposing immature CRs and mature CRs to 30 μg/mL and 50 μg/mL BFA for 24 h (n = 5, 4 biological replicates for immature, mature CRs, respectively). Two-tailed t-test, mean ± SD. ***P = 2.49 × 10–5 (30 μg/mL), 9.14 × 10–8 (50 μg/mL); ns not significant (P = 0.98, 0.06 for 30, 50 μg/mL, respectively). Light micrographs of mycelia and CRs after applying 50 µg/mL BFA. Scale bars = 50 µm. Source data are provided as a Source Data file. Data shown are representatives from experiments performed in triplicate.

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