Fig. 5: Localization of DdSnc1 and DdSnc2 fused with RFP and GFP, respectively.

a, e Hyphal tips. The dotted white line denotes the hyphal tip. b, f Incompletely formed CRs (partially completed fusion was noted by black arrow). c, g Immature CRs with completed fusion (noted by black arrow). d, h Mature CRs. The dotted white circle in (b–d, f–h) denotes the inner side of CR. The scale bars for (a–h) are 6 µm. i Levels of DdSnc2 transcripts in two ΔDdSnc1 mutants during vegetative growth (n = 4 independent experiments). Two-tailed t-test, mean ± SD. ***P = 0.00011 (ΔDdSnc1–8), 7.12 × 10–5 (ΔDdSnc1–11). j Levels of DdSnc1 transcripts in two ΔDdSnc2 mutants during vegetative growth (n = 4 independent experiments). Two-tailed t-test, mean ± SD. ***P = 3.03 × 10–6 (ΔDdSnc2–7), 2.93 × 10-8 (ΔDdSnc2−53). Source data are provided as a Source Data file. The data shown here are representatives from duplicated experiments.