Fig. 7: Variable engraftment rate of BCLM PDOs in the orthotopic site. | Nature Communications

Fig. 7: Variable engraftment rate of BCLM PDOs in the orthotopic site.

From: Genomic profiling and pre-clinical modelling of breast cancer leptomeningeal metastasis reveals acquisition of a lobular-like phenotype

Fig. 7

Female NSG mice were inoculated with mChLuc2-labelled PDOs as follows. a Non-dissociated BCLM PDOs (~ 1 × 106 cells) were injected transcutaneously into the 4th mammary fat pad (unilateral), n = 4–6 mice per PDO. Upper panel, tumour size was measured bi-weekly. Middle panel, mammary fat pad tumours stained for H&E, lamin A/C, ER and E-cadherin. Scale bar, 100 µm. Lower panel, bar charts show percentage of mice with metastatic dissemination in individual organs. Pale bars indicate % tissues with single or small cell clusters (1–10 cells), dark bars indicate tissues with metastatic deposits ( > 10 cells), source data are provided as a Source Data file. b 4 × 104 dissociated KCL320 PDO cells per gland were inoculated via the mammary gland intraductal (MIND) route into the 3rd, 4th and 5th mammary glands, bilaterally (6 glands/mouse; n = 6 mice). Mice were IVIS imaged on day 0 and day 165 (endpoint). c 2 × 106 dissociated KCL320 PDO cells injected intraperitoneally (n = 4 mice) with IVIS imaging at day 0 and day 81 (endpoint). Created with BioRender.com.

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