Fig. 5: Budding of blisters.
From: Patterning and dynamics of membrane adhesion under hydraulic stress
a Confocal images of a GUV-SLB adhesion patch (xy and xz) at 4 mol% biotinylated lipids, at t = 0 (i), 140 s (ii), and 25 min (iii) following a 100 mM osmotic shock. b 3D confocal images of two adhered vesicles at 4 mol% biotinylated lipids and subjected to 100 mM osmotic shock at 30 (i) and 470 (ii) s after the shock. The insets show corresponding 2D cross-sections of the GUV-GUV adhesion patch at 0 (i) and 470 (ii) s. c Change in adhesion area and linker density (quantified as mean NAV fluorescent intensity in the adhesion zone) over time for two adhered vesicles at 4 mol% biotinylated lipids, subjected to 100 mM osmotic shock. The values in both plots are normalized to the initial values, where t = 0 is the time when imaging starts (approximately 30 s after the osmotic shock). The mean NAV fluorescent intensity is corrected for photobleaching and the data points correspond to 3 different z planes for each time frame.
