Fig. 3: Self-incompatibility was not restored after the introduction of the repaired recessive AhSCR-D.

a SCR-D expression in the anther cDNAs of Arabidopsis halleri bearing haplogroup D (Ahal-D), WT Potter, and four transgenic Potter lines (Pot_SCRD). ACTIN was amplified as a quantitative control. b Siliques of WT Potter and transgenic Potter lines. Scale bar = 10 mm. c Comparison of silique length from self-pollination of WT Potter and transgenic Potter lines. The silique length of transgenic lines was not significantly different from that of the WT. d Comparison of the number of seeds per silique from self-pollination of WT Potter and transgenic Potter lines. The length of silique (c) or the number of seeds per silique (d) in the transgenic lines was not significantly different from that in the WT (one-way ANOVA and Tukey HSD test, neach line = 5, see Supplementary Table 7 for exact P-values). Boxplots c and d show center line: median; box limits: upper and lower quartiles; whiskers: within 1.5 times the interquartile range; dots: data points. Source data underlying c and d are provided as a Source data file.