Fig. 4: JHU083 treatment elicits early recruitment of T-cells in the lungs.

As described in Fig. 2a, Mtb-infected female 129S2 mice (n = 5/group) were treated with JHU083, RIF, and PBS daily starting on day 1 post infection. Mice were sacrificed on week 2 and week 5, and the lungs were harvested. Single cell suspensions of the lungs from the three treatment groups were stained with appropriate antibodies and analyzed using multicolor-flow cytometry. We found differences in the frequencies or geometric mean fluorescence intensities (gMFI) for: (a) CD4⁺ T-cells, (b) TNFα expression on activated (CD44⁺) CD4⁺ T-cells, (c) IFNγ expression on activated (CD44⁺) CD4⁺ T-cells, (d) IL-10 expression on activated (CD44⁺) CD4⁺ T-cells, (e) naïve CD4⁺ T-cells, (f) follicular helper (BCL6⁺) CD4⁺ T-cells, (g) CD62L expression on CD8⁺ T-cells, (h) BCL6 expression on CD8⁺ T-cells, (i) Klrg1 expression on CD4⁺ T-cells, (j) Klrg1 expression on CD8⁺ T-cells, (k) mature B-cells (CD19⁺ CD27^- CD138^-), and (l) memory B-cells (CD19⁺ CD27⁺ CD138^-). The X-axis indicates the lung harvest timepoint. All T-cell data (a–j) came from the lungs harvested at week 2 (W2) post-infection/treatment while B-cell data (k–l) was generated from lungs harvested at week 5 (W5) post-infection/treatment. For all graph panels, n = 5/group except RIF-treated group in graph (e–j) due to loss of a sample during processing (n = 4). Data were plotted as mean ± SEM and are shown as the frequency of CD45⁺ population. gMFI was mostly used for low abundance cell surface markers and transcription factors. Statistical significance was calculated using a two-tailed student t-test considering unequal distribution. The exact p-values are provided in the Source Data file. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. NS stands for non-significant change, p-value was >0.05. The experiment was repeated two times.