Fig. 3: The Golgi membrane tail-stretching processes were impaired in myosin xik xi1 xi2 triple mutants (xi3KO).

a Time-lapse images showing the Golgi membrane tail-stretching processes labeled by GFP-STL2 in the control and xi3KO cells. Representative events of successful rupture, Golgi reversal and tail-end retraction were shown in the left, middle and right panels, respectively. Arrows indicated the Golgi, while the arrowheads indicated the end of the Golgi tail. The progress of the membrane tail-stretching events at the indicated time points was schematically presented above the images. Bars = 2 μm. b Percentage of different groups of Golgi membrane tail-stretching events in the control and xi3KO cells. Values are mean ± SE. n = 24 cells from 12 WT seedlings examined over 3 independent experiments; n = 27 cells from 12 xi3KO seedlings examined over 3 independent experiments. *P value < 0.05; ns, not significant; two-sided Student’s t test. c–e Quantification of the maximal tail length (c), the displacement of the tail ends (d) and the tail stretching time (e) during the Golgi membrane tail-stretching processes as exemplified in (a). Values are mean ± SD. In total, 70 membrane tail-stretching events in 11 control cells and 67 membrane tail-stretching events in 10 xi3KO cells were quantified. *P value < 0.05; **P value < 0.01; ***P value < 0.001; ns, not significant; two-sided Student’s t test.