Fig. 6: Phase separation of FUS LC domains on membranes.
From: Transmembrane coupling of liquid-like protein condensates
a Representative microscopic images showing the fusion of protein-rich regions. White arrowheads indicate fusion events. b Representative microscopic images showing transbilayer domain coupling. Scale bars, 5 µm. c Relative intensity profiles along the dotted lines (from A to B) in the merged channel in b, where green and red lines represent the intensity from the protein and lipid channels, respectively. See the caption of Fig. 3c for the definition of relative intensity. 500 nM of his-FUS LC, labeled with Atto 488, was used. Membrane composition: 80 mol% DOPC, 20 mol% DGS-Ni-NTA, and 0.5 mol% Texas Red-DHPE. Buffer: 25 mM HEPES, 150 mM NaCl, pH 7.4. Scale bars, 10 µm. Source data are provided as a Source Data file.
