Fig. 5: Water transport phenotype for ADPKD drug discovery applications. | Nature Communications

Fig. 5: Water transport phenotype for ADPKD drug discovery applications.

From: A semiconductor 96-microplate platform for electrical-imaging based high-throughput phenotypic screening

Fig. 5

a, b A set of 10 compounds were tested against the water transport phenotype exhibited in MDCK (canine kidney) cells; Supplementary Movie 2 shows the full 96-well CMOS microplate movie. Select time point electrochemical images for 1 of 3 replicates for Alisertib (1 µM) and Bosutinib (10 µM) are shown in (a) with a colormap in (b). Domes were only observed for tissues treated with Alisertib. c Line traces show Alisertib accelerated and Bosutinib delayed the water transport detachment (LF 16 kHz) with respect to the control; mean ± s.d. for 3 wells per condition. The differential results match animal study results (Main text) for autosomal dominant polycystic kidney disease (ADPKD), suggesting the water transport phenotype could be predictive of ADPKD efficacy. Source data are provided as a source data file.

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