Fig. 3: MatP relocalization precedes nucleoid constriction.
From: Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli
a Line profile plots of HU-mCherry signal on different frames with corresponding images. Increasing darkness of blue indicates later times. The black line indicates the profile from the frame at which the nucleoid constricts. The nucleoid is considered constricted if the relative depth of the dip in the signal (if one exists) is greater than a threshold chosen to account for normal variation in the signal (see Supplementary Fig. 4a, b). The threshold for the frame indicated by the arrow is shown in red. The time of stable nucleoid constriction TNucleoid is the earliest frame after which a dip greater than the threshold is observed in the nucleoid signal for the rest of the cell cycle. b Distribution of the time of MatP centralisation, TMatP (mean ± sd = 49.5 ± 28.4 min) and stable nucleoid constriction, TNucleoid (94.2 ± 43.7 min) along with the time difference between the two events (51.5 ± 38.1 min) as in Fig. 1. The horizontal lines indicate the median values of 45 and 90 min for TMatP and TNucleoid respectively. The dot indicates the median (40 min) of the paired difference TNucleoid−TMatP. Data as in Fig. 2. See also Supplementary Figs. 2, 4. Source data are provided as a source data file.
