Fig. 10: HUVEC^vegf165+-laden hydrogel ameliorates oxygenation and inflammation of diabetic wounds.

A Proteome profiler rat cytokine array of diabetic wounds on day 7 with different treatments. B Quantitative analysis of the signal intensity of cytokines. n = 2 dots for each cytokine in the membrane. C IVIS spectrum of luminescent oxygen probe for the detection of oxygen contents of diabetic wounds on day 4. i: hydrogel; ii: HUVEC^vector-laden hydrogel; iii: HUVEC^vegf165+-laden hydrogel. D Comparison of HIF-1ɑ transcriptional level among the diabetic wounds with different treatments on days 3, 7, and 14. n = 5 rats/group. E Comparison of MDA concentration among the diabetic wounds with different treatments on days 3, 7, and 14. n = 5 rats/group. F Comparison of TNF-ɑ production among the diabetic wounds with different treatments on days 3, 7, and 14. n = 5 rats/group. G Therapeutic mechanism of wound healing by HUVEC^vegf165+-laden hydrogel. VEGF 165 was continuously released from the HUVEC^vegf165+-laden hydrogel to rescue HG-induced vascular endothelial cell death by inhibiting mitochondrial oxidative stress, thus improving tissue angiogenesis and oxygenation, and creating an eligible microenvironment for diabetic wound healing. This schematic diagram was created with BioRender.com and has been granted a publication license. The p values in the figures (D–F) are determined by two-way ANOVA followed by Tukey’s multiple comparisons test. Data are presented as mean ± SD. Source data are provided as a Source Data file.