Fig. 8: Histological analysis of the healing process of diabetic wounds with different treatments.
From: A click chemistry-mediated all-peptide cell printing hydrogel platform for diabetic wound healing
A CK14/CK10 immunofluorescence (IF) staining of the epithelial layer. $: fibrous capsule; #: regenerated granulation tissue; &: edges of normal skin; dotted line: boundary separating normal skin and wound; dot-and-dash line: extended epithelial layer that covers the wound bed. n = 5 rats/group. B CK14 IF staining of hair follicles. Φ: epithelial layer; arrows: hair follicles. VS, visual field. n = 10. C Picrosirius red staining of collagen in the wounds. n = 10. D Vascular structure stained with CD 31 and ɑ-SMA. Arrows: microvessels. n = 10. E Staining of proliferative cells by Ki67. n = 10. F Evaluation of cell metabolic rate by PGC-1ɑ staining. n = 10. For A–F, i: representative tissue staining images; ii: quantitative analysis of corresponding wound images on day 7; iii: quantitative analysis of corresponding wound images on day 14. For B–F, n = 10 because two random visual fields were selected for each tissue sample harvested from five rats in each group. The p values in each figure (ii and iii) are determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data are presented as mean ± SD. ns, not significant. Source data are provided as a Source Data file.
