Fig. 4: Myeloid cell-specific ITGA5 loss does not affect infiltration of the infarcted heart with myeloid cells, macrophages, neutrophils, and T lymphocytes. | Nature Communications

Fig. 4: Myeloid cell-specific ITGA5 loss does not affect infiltration of the infarcted heart with myeloid cells, macrophages, neutrophils, and T lymphocytes.

From: Protective effects of macrophage-specific integrin α5 in myocardial infarction are associated with accentuated angiogenesis

Fig. 4

Flow cytometry was used to compare the percentage and absolute number of cells in the infarct, 7 days after myocardial infarction. The gating strategy is shown in Supplementary Fig. 7. DAPI was used to label live cells (DAPI-cells). Myeloid cells were identified as DAPI-/CD45 + /CD11b+ cells (A), macrophages were labeled as DAPI-/CD45 + /CD11b + /Ly6G-/CD64 + /MerTK+ cells (B), whereas T cells were identified as DAPI-/CD45 + /CD11b-/CD3e+ cells (C). Quantitative analysis showed no significant effects of myeloid cell-specific ITGA5 loss on myeloid cell (D, E), macrophage (F, G), neutrophil (H, I), and T cell (J, K) numbers in the infarct (p = NS, n = 5 biologically independent experiments/group). Panels D, F, H, and J show the percentage of each population in relation to all live cells (DAPI-cells), whereas panels E, G, I, and L show the absolute number of cells per mg of myocardial tissue. Data are shown as mean values +/- SEM, Statistical analysis was performed using unpaired two-tailed Student’s t test. Source data are provided as a Source Data file. NS no significance.

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