Table 1 B. subtilis strains used in this study

From: Direct comparison of spatial transcriptional heterogeneity across diverse Bacillus subtilis biofilm communities

Strain

Relevant genotype or isolation source

Construction or Referencea

NDmed

Non-Domesticated wild type strain

15

NDmed-GFP GM3649

NDmed amyE::Phyperspank-gfpmut2 (spec)

16

BSB168

trp+ derivative of 168

63,64

BBA093

BSB168 PackA-gfpmut3 (spec)

Matthieu Julesb

BBA0231

BSB168 Phag-gfpmut3 (spec)

Matthieu Julesb

BBA0290

BSB168 PbslA-gfpmut3 (spec)

Matthieu Julesb

BBA0428

BSB168 PsrfAA-gfpmut3 (spec)

Matthieu Julesb

BBA9006

BSB168 PgapB-gfpmut3 (spec)

Matthieu Julesb 65,

GM3346

NDmed Phag-gfpmut3 (spec)

BBA0231→NDmed

GM3348

NDmed PackA-gfpmut3 (spec)

BBA0093→NDmed

GM3378

NDmed PgapB-gfpmut3 (spec)

BBA9006→NDmed

GM3401

NDmed PbslA-gfpmut3 (spec)

BBA0290→NDmed

GM3402

BSB168 PepsA-gfpmut3 (spec)

pBSB2epsA → BSB168

GM3403

NDmed PsrfAA-gfpmut3 (spec)

BBA0428 →NDmed

GM3423

NDmed PepsA-gfpmut3 (spec)

GM3402→NDmed

GM3461

BSB168 PypqP-gfpmut3 (spec)

pBSB2ypqP → BSB168

GM3476

NDmed PypqP-gfpmut3 (spec)

GM3461→NDmed

GM3816

NDmed PctaA-gfpmut3 (spec)

pBSB2ctaA→NDmed

GM3820

NDmed PnarG-mCherry (cm)

pBSB8narG→NDmed

GM3823

NDmed PskfA-mCherry (cm)

pBSB8skfA→NDmed

GM3838

NDmed PcomGA-gfpmut3 (spec)

pBSB2comGA→NDmed

GM3841

NDmed PaprE-mCherry (cm)

pBSB8aprE→NDmed

GM3859

NDmed PcggR-mCherry (cm)

pBSB8cggR→NDmed

GM3862

NDmed capE-mCherry (cm)

pBSB8capE→NDmed

GM3864

NDmed PspoIIGA-mCherry (cm)

pBSB8spoIIGA→NDmed

GM3867

NDmed PspoVC-mCherry (cm)

pBSB8spoVC→NDmed

GM3874

NDmed PtapA-mCherry (cm)

pBSB8tapA→NDmed

GM3900

NDmed PgapB-gfpmut3 (spec)/PcggR-mCherry (cm)

GM3859 → GM3378

GM3903

NDmed PaprE-mCherry (cm)/PackA-gfpmut3 (spec)

GM3841 → GM3348

GM3907

NDmed PnarG-mCherry (cm)/PctaA-gfpmut3 (spec)

GM3820 → GM3816

GM3912

NDmed PskfA-mCherry (cm)/PcomGA-gfpmut3 (spec)

GM3838 → GM3823

GM3924

NDmed PtapA-mCherry (cm)/Phag-gfpmut3 (spec)

GM3346 → GM3874

  1. aarrows indicate transformation of pointed strain with indicated plasmid or chromosomal DNA of indicated strain.
  2. bUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350 Jouy-en-Josas, France.
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