Fig. 5: Dissociation of #4C214A and #4VL tetramers into monomers. | Nature Communications

Fig. 5: Dissociation of #4C214A and #4VL tetramers into monomers.

From: Structural and thermodynamic insights into antibody light chain tetramer formation through 3D domain swapping

Fig. 5

a Plot depicting the relationship between ΔG° and temperature for #4C214A, including the fitted curve based on Eq. (4). b Thermodynamic parameters for the monomer-tetramer equilibrium of #4C214A at 25 °C. c A plot similar to Fig. 5a for #4VL. d A table corresponding to Fig. 5b for #4VL. e Time-course tracking of the tetramer ratio change in #4C214A along with the corresponding exponentially fitted curves. f Rate constants for #4C214A, illustrating the dissociation from tetramers to monomers at different temperatures. g Graphs similar to Fig. 5e for #4VL. h A table corresponding to Fig. 5f for #4VL. i CD spectra of #4VL at different concentrations: 100 µM (red) and 10 µM (blue). Source data are provided as a Source Data file. Each analysis has been performed at least 3 times.

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