Fig. 7: Administration of rmSLIT2 increased activity in aged mice.
From: Mid-old cells are a potential target for anti-aging interventions in the elderly
a PBS (control) or rmSLIT2 (2 μg/injection) was intraperitoneally injected twice weekly for 5 weeks in 23-month-old male mice (n = 5; PBS, n = 5; rmSLIT2, respectively) (upper panel). The body weight (middle panel) and activity in the cage (lower panel) were evaluated at the experimental beginning and endpoint. b The hanging ability of the rmSLIT2 or PBS-treated mouse was compared (left panel). The quantification data is shown in the right panel. c The muscle weight of the rmSLIT2 or PBS-treated mouse was compared (left panel). Laminin IF staining (middle panel). The tibialis anterior and gastrocnemius muscle were transversely sectioned to measure the area of muscle fibers (right panel). Representative images of laminin (green) and DAPI (blue) staining (middle panel). p21Waf1 (d) and SOX2 (e) expression were analyzed by IHC in stromal region of esophagus, stomach, small intestine, colon, lung, and skin, respectively (n = 5; PBS, n = 5; rmSLIT2). Each protein expression status was indicated positive cells number/100 stromal cells. The p values of (a–e) are obtained from the one-tailed Mann–Whitney U test. The graphs in (a–e) were represented as mean ± SD.
