Fig. 4: Dietary 3HAA extends lifespan, improves health metrics, and promotes a less inflammatory immune cell profile in mice. | Nature Communications

Fig. 4: Dietary 3HAA extends lifespan, improves health metrics, and promotes a less inflammatory immune cell profile in mice.

From: On the benefits of the tryptophan metabolite 3-hydroxyanthranilic acid in Caenorhabditis elegans and mouse aging

Fig. 4

a C57BL/6J mice fed chow supplemented with 312.5 ppm (low dose, L; N = 6) or 3125 ppm (high dose, H; N = 7) 3HAA starting at 27 months of age live longer than mice fed control diet (C; N = 8). Kaplan-Meier survival curves show remaining lifespan following introduction of the 3HAA diet. Colored text indicates change in mean remaining lifespan relative to control. b Neither 3HAA supplemented diet affected body weight. c Dietary 3HAA supplementation increases 3HAA concentration in serum and urine. d Mice on low dose 3HAA diet trended toward increased all paw grip strength after 13 weeks. Dietary 3HAA alters circulating immune cell profiles after 10 weeks: e shifting myeloid cells away from neutrophils/granulocytes and toward monocytes, f decreasing or preventing an increase in natural killer (NK) within several sub-lineages, g preventing an increase in double negative (DN) T cells, and h creating a trend toward reducing B cell counts. #p < 0.1, †, *p < 0.05, ‡, **p < 0.01, ***p < 0.001 vs. control diet (Wald test in Cox regression with weighted estimation, panel a; two-sided Welch’s t test, panels b–h). For panel d, *, **, *** indicate significance vs. Week 0; †, ‡ indicate significance vs. control diet. Error bars indicates standard error of mean. Summary statistics are provided in Supplementary Data 1 and source data are provided in Source Data.

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