Fig. 6: ampC-dependent beta-lactam resistance conferred by the V111D substitution in fumarate reductase subunit FrdD. | Nature Communications

Fig. 6: ampC-dependent beta-lactam resistance conferred by the V111D substitution in fumarate reductase subunit FrdD.

From: Global pathogenomic analysis identifies known and candidate genetic antimicrobial resistance determinants in twelve species

Fig. 6

a Enrichment for resistant over susceptible genomes from the presence of frdD mutations resulting in the V111D substitution across 14 beta-lactam drugs, based on log2 odds ratio (LOR). Number of genomes with AMR data is shown for each drug. b Overlap between the frdD coding region and ampC promoter in E. coli BW25113 and predicted ampC transcription rates for various frdD mutations affecting V111. c Maximum cell density (OD600) achieved by ampC and frdD mutants under increasing ampicillin (AMP) concentrations in rich (CA-MHB) or minimal (M9) media. Error bars indicate standard deviations centered around means from biologically independent triplicates. Six genotypes were tested, from combinations between three possible codons in frdD affecting V111 in either the BW25113 wildtype (WT) or corresponding ampC knockout (KO) strain. Starred case indicates growth was not observed until at least 8 h after inoculation for all replicates.

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