Fig. 2: Local CSF1 restores depleted RMs. | Nature Communications

Fig. 2: Local CSF1 restores depleted RMs.

From: DPPIV+ fibro-adipogenic progenitors form the niche of adult skeletal muscle self-renewing resident macrophages

Fig. 2

a The amount of CSF1 detected by ELISA in skeletal muscle at different time points after CSF1R inh/wd (each dot represents one mouse, data were pooled from ≥3 experiments). b The amount of CSF1 detected by ELISA in the blood within three days after withdrawal of CSF1R inhibition or the control diet (each dot represents one mouse, data were pooled from 2 experiments, unpaired t-test). c Expression of Csf1 in different muscle resident cell populations at different time points after CSF1R inh/wd assessed by droplet digital PCR (ddPCR, n = 8 mice for FAPs, n = 5 mice for the other populations, data were pooled from ≥3 experiments, Brown-Forsythe and Welch ANOVA tests). d The number of TIM4- and TIM4+ RMs detected by flow cytometry in skeletal muscle from tamoxifen (TAM)-induced PdgfrαCreERT2 × Csf1flox/null or transgene (Tg) controls following CSF1R inh/wd (n = 11 for control and n = 10 for CSF1R inh/wd, data were pooled from ≥3 experiments, unpaired t test with Welch’s correction).

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