Fig. 1: Overview of mtLSU biogenesis intermediates.
From: Structural insights into the role of GTPBP10 in the RNA maturation of the mitoribosome
a Cryo-EM densities of the isolated mtLSU maturation intermediates with the associated biogenesis factors colored. The colors of the protein names and respective densities are matched. b Overview of the structural model of intermediate 1 containing the GTPases GTPBP10 (yellow), GTPBP7 (blue), the module of MALSU1, L0R8F8, mtACP (shades of violet), and the dimer of MTERF4 (yellowgreen) and NSUN4 (cyan). The ribosomal RNA is shown as surface representation whereas all proteins are shown as cartoons. Prominent ribosomal elements are labeled including the sarcin-ricin loop (SRL), the peptidyl-transferase center (PTC), and ribosomal rRNA helix H89 (H89). c GTPases GTPBP10 and GTPBP7 are shown in isolation with important ribosomal RNA elements highlighted. Both GTPases likely contain the non-hydrolysable nucleotide analog Guanosine-5’-[(β,γ)-imido]triphosphate (GMPPNP) in their active sites.
