Fig. 4: Interactions of GTPBP7 with GTPBP10 and ribosomal RNA.
From: Structural insights into the role of GTPBP10 in the RNA maturation of the mitoribosome
a Overview of GTPBP7 and GTPBP10 in the context of adjacent mitoribosomal RNA elements. SRL = sarcin-ricin loop (b, c) Two views of the interaction interface between GTPBP10 and GTPBP7 are shown with the experimental EM density. Interacting residues are highlighted. The sharpened, supersampled EM density is shown at 2.3 σ. d Interface of GTPBP7 and ribosomal RNA with the corresponding experimental EM density at 2.3 σ. e Overlay of the rRNA of intermediate 1 (light blue, cartoon) and an earlier maturation intermediate (gray, surface, PDB 5OOM)7. GTPBP7 is shown as silhouette at its binding site in intermediate 1 (blue). rRNA elements adjacent to GTPBP7 are not visible in the earlier maturation intermediate. f The location of histidine 34 (H34) of GTPBP7 in relation to the ribosomal A loop is depicted. EM density is shown at 2 different thresholds (2.3 σ (gray), 3.2 σ (red)).
