Fig. 2: Molecular tensions measured by the TS temporally correlate to tractions from TFM.

a TS cell on a circular pattern, showing the RFP of the sensor b and the associated FRET E map. Scale bar is 5 µm. Experiments were repeated eight times independently. c mean FRET E (black dots) and traction (orange dots) over time for a 10 nM Calycluin-A treated TS cell. d Traction magnitude and FRET E over time for a 10 μM Blebbistatin-treated TS cell (e) corresponding traction vectors (\(\vec{{{{{{\rm{T}}}}}}}\)) on the PAA gel before and after addition of Calyculin-A. Grid size is 6.4 μm. f Traction and FRET E over time for a 10 nM Calycluin-A treated CTS cell. g FRET E for TS, CTS and drug treated cells. CTS (n = 11 cells, N = 53 measurements at different times), for TS (n = 12 cells N = 77 measurements at different times) for TS+Calyc. (n = 12 cells N = 87 measurements at different times), for TS+ Blebb. (n = 7 cells N = 87 measurements at different times). Tukey’s test was used for significance. Two-sided t-test with p(CTS and TS) = 0.0176, **** is p < 0.0001. h corresponding traction vectors (\(\vec{{{{{{\rm{T}}}}}}}\)) on the PAA gel before and after addition of Blebbistatin. Grid size is 6.4 μm. i <FRET E>-\( < |\vec{{{{{{\rm{T}}}}}}}| > \) relationship after 10 nM Calyculin-A (n = 12, ns regression p-value = 0.1579) and 10 μM blebbistatin (n = 7, ns regression p-value = 0.1192) treatment of CTS cell over time. Two-tailed t-test was used for significance. ns is non-significant. Data are presented as mean values ±SD. j <FRET E>-\( < |\vec{{{{{{\rm{T}}}}}}}| > \) relationship for Calyculin-A (n = 12 cells N = 77 measurements at different times) and Blebb. (n = 7 cells N = 87 measurements at different times) treated TS cells. Two-tailed t-test was used for statistical significance with p < 0.0001 for each line. The slopes are significantly p < 0.0001 different (F = 21.00). Data are presented as mean values ±SD. Source data are provided as a Source Data file.