Fig. 3: Adaptive laboratory evolution of the V. natriegens strains further enhances formate tolerance and metabolism. | Nature Communications

Fig. 3: Adaptive laboratory evolution of the V. natriegens strains further enhances formate tolerance and metabolism.

From: Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery

Fig. 3

a Flowchart for the adaptive laboratory evolution of the V. natriegens S-TCA-1.0. S-TCA-2.0 was the final evolved strain with significantly enhanced formate metabolism ability in LBv2 medium. The lightning symbols represent UV mutagenesis, which was carried out every three passages, aiming to accelerate the strain mutation and evolutionary efficiency. b Evolution trajectory of S-TCA-1.0 to obtain the S-TCA-2.0 strain with gradually enhanced formate utilization. LBv2 medium was used with an increasing sodium formate (HCOONa·2H2O) concentration from 20 to 140 g · L‒1 (192 to 1346 mM equivalent formate). Triangles indicate the bacterial biomass of each passage; the same colors represent passages with the same formate concentration. c Validation of the growth and formate consumption of S-TCA-2.0 within 24 h using the LBv2 medium initially supplemented with 85 g · L‒1 sodium formate (817 mM equivalent formate). Data are presented as the mean ± SD (n = 3 biologically independent samples). d The nine mutated genes occurred in all the three isolates of the S-TCA-2.0 stain. Source data are provided as a Source Data file.

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