Table 1 In vivo limiting dilution assays of SPINK1 knockdown

From: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma

Huh7

Tumor incidence

Average latency (Days)

Estimated TIC frequency

Primary implantation

Cell numbers

5k

10k

50k

5k

10k

50k

 

NTC

5/8

4/8

6/8

31.9

32.6

25

1/18262

SH2

1/8

1/8

0/8

34.1

34.8

-

1/256230 (p = 8.36e-6)

SH3

1/8

3/8

3/8

34.1

33.3

33.6

1/59524 (p = 0.0444)

Secondary implantation

Cell numbers

5k

10k

 

5k

10k

  

NTC

10/14

13/14

 

20.3

19.1

 

1/3897

SH2

3/14

6/14

 

24.4

22

 

1/18836 (p = 5.63e-5)

SH3

10/14

7/14

 

19.8

22.5

 

1/8271 (p = 0.0437)

  1. In vivo limiting dilution assays, in serial passage, for evaluating tumor-initiating cells (TICs) frequency of Huh7 cells with either non-target scrambled control (NTC) or SPINK1 knockdown lentiviral transduction (SH2 and SH3). Tumor incidence, average latency and estimated tumor-initiating frequency data. n = 4 mice for primary implantation; n = 7 mice for secondary implantation. Significance was calculated by one-sided Pearson’s χ2 test comparison to NTC.
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