Fig. 5: POL2A mutation normally delays S-phase progression but promotes it in topology stress.
a S-phase progression of root tips measured by double-labeling strategy in 4-day-old Col-0, atm, asr20, pol2aL473F. 4-day-old seedlings were firstly pulsed with 10 μM EdU for 15 min, a second 50 μM BrdU pulse for 15 min with DMSO or 1 μM CPT, as the schematic workflow shown in the upper left. A diagram of cell types labeled by EdU or BrdU in the cell cycle was shown in the upper right. b Percentage of double-labeled (EdU and BrdU), single-labeled (EdU) nuclei related to Fig. 5a, >300 nuclei from 3 roots were scored for each sample (one-way ANOVA). c S-phase progression of root tips measured by double-labeling strategy in 4-day-old seedlings. After firstly pulsing with 10 μM EdU for 15 min, a 1 h 20 μM thymidine pulse with DMSO or 1 μM CPT, and then a second 50 μM BrdU pulse for 15 min with DMSO or 1 μM CPT, as the schematic workflow shown above. d Percentage of double-labeled (EdU and BrdU), single-labeled (EdU) nuclei related to Fig. 5c, >300 nuclei from 2 or 3 roots were scored for each sample. e S-phase progression of root tips measured by double-labeling strategy in 4-day-old seedlings. After firstly pulsing with 10 μM EdU for 15 min, a 2 h 20 μM thymidine pulse with DMSO or 1 μM CPT, and then a second 50 μM BrdU pulse for 15 min with DMSO or 1 μM CPT treatment, as the schematic workflow shown above. f Percentage of double-labeled (EdU and BrdU), single-labeled (EdU) nuclei related to Fig. 5e, >300 nuclei from 2 or 3 roots were scored for each sample. Source data are provided as a Source Data file.
