Fig. 6: Effects of ATM and POL2A in DNA replication and DNA damage. | Nature Communications

Fig. 6: Effects of ATM and POL2A in DNA replication and DNA damage.

From: DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in Arabidopsis

Fig. 6

a Immunolabeling of root tips nuclei from 5-day-old Col-0, atm, and asr20 treated with DMSO or 1 μM CPT for 2 h using EdU and γH2AX antibody. Yellow triangle: EdU+γH2AX+ nuclei; white triangle: EdU+γH2AX- nuclei; yellow arrow: EdU-γH2AX+ nuclei; white arrow: EdU-γH2AX- nuclei. Scale bar: 10 μm. Experiments were repeated three times with similar results. b Strategies of cell type classification according to EdU and γH2AX intensity. c Percentage of DNA damage occurring during replication corresponding to Fig. 6a. More than 150 nuclei were scored for each sample.

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