Fig. 7: POL2A and ATM guard the fidelity of DNA replication. | Nature Communications

Fig. 7: POL2A and ATM guard the fidelity of DNA replication.

From: DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in Arabidopsis

Fig. 7

a Ribonucleotide incorporation detection with RNase HII cleavage products of genomic DNA extracted from Col-0, atm, asr20, and pol2aL473F treated with DMSO or 30 nM CPT for 7 days. The rNTPs incorporation ratio was elevated in the asr20 genome after CPT treatment as 2–5 kb DNA fragments (dashed red line) were predominantly detected. Experiments were repeated three times with similar results. b Densitometry plot of lanes corresponding to Fig. 7a. c Root phenotypes of 7-day-old Col-0, atm, atrnh2a/pol2aL473F, atrnh2a, and pol2aL473F treated with DMSO or 30 nM CPT. Scale bar: 1 cm. d The quantitative data of root lengths for Fig. 7. Lines represent mean ± SD, n = 16 (one-way ANOVA). Source data are provided as a Source Data file.

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