Fig. 3: Characterization of organ-selective lipid nanoparticle systems.
a Structural formula and schematic diagram of each component of lung-selective and liver-selective LNPs. b Schematic diagram of lung-selective and liver-selective LNPs. c Transmission electron microscopy (TEM) results: upper image (Lung-LNPs) and lower image (Liver-LNPs). d, e Dynamic light scattering (DLS) results for liver-selective (d) and lung-selective LNPs (e). f Particle size change for two particles in PBS, data are shown as the mean ± SD, n = 3 biologically independent samples. g, h Zeta potential analysis of liver-selective (g) and lung-selective LNPs (h), n = 3 independent experiments. i, In vivo imaging of liver-selective and lung-selective LNPs@mRNALuci at different time points, n = 4 for each LNPs@mRNALuci at different time points. j, k Visceral imaging of liver-selective and lung-selective LNPs@mRNALuci. The organs are heart (h), lung (Lu), liver (Li), stomach (St), intestine (I), kidney (K), and spleen (Sp). l The statistical results of luminescence intensity from mice inoculated with liver-selective and lung-selective LNPs@mRNALuci at different time points, the ROI of liver-selective LNP statistics was the liver, and the ROI of lung-selective LNP statistics was the lungs, data are shown as the mean ± SD, n = 4 independent samples. m Imaging liver tissue or lung tissue of mice inoculated with liver-selective and lung-selective LNPs@mRNAeGFP at 24 h post injection. Source data are provided as a Source data file.
