Fig. 7: Phenotypic comparison of B4/H24RxC and B5/H24RxC clones.

a Growth curves in half strength LB for strains of both clones. Strain ATCC 25922 was included as a growth control. Data are shown as mean ± SD from n = 3 biological replicates. b Doubling time in half strength LB for isolates of both clones. Strain ATCC 25922 was included as a growth control. Data are shown as mean ± SD from n = 3 biological replicates. Statistical difference was assessed with two-tailed unpaired Student’s t test. c, d qPCR-based competition assay for strains of both clones. The figures show the relative quantity ratio of gene fyuA in B5/H24RxC strains to gene yodB in B4/H24RxC strains 005828 and 045869. Data are shown as mean ± SD from n = 3 biological replicates. Statistical difference was assessed with two-tailed unpaired Student’s t test. e Survival curves for wax moth larvae (G. mellonella) infected with ~2 × 10⁶ CFU of different isolates in the B4/H24RxC and B5/H24RxC clones. Hypervirulent Klebsiella pneumoniae strain K1088⁶⁵ and hypervirulent Acinetobacter baumannii strain AB5075⁶⁶ were used as positive controls while Acinetobacter baumannii ATCC 19606 and PBS were used as negative controls. The curves represent the mean of three biological repeats. f The ability to utilise different iron sources by B4/H24RxC and B5/H24RxC clones. Green circles indicate growth and the numbers inside show the lowest tested concentration of the iron sources needed for the isolates to grow. Grey circles indicate no growth was observed at any concentration of the iron sources used. n = 2 biological independent experiments with the same result. Source data are provided as a Source Data file.