Fig. 6: Characterization of Rac-dependent Arhgef11 and Arhgef12 plasma membrane recruitment.

a Schematic representation of the Arhgef11 constructs that were used investigate the mechanism of Rac-stimulated plasma membrane recruitment. b–f Measurement of full length or mutant Lbc-type GEF plasma membrane recruitment during Rac activation in A431 cells that co-express PA-Rac1 (n = 3 independent experiments). b, d, e Measurement of recruitment kinetics. c, f Quantification of recruitment in the 25 s time frame during photoactivation (early response) or 1 min after photoactivation (late response). d Measurement of Arhgef11/12 plasma membrane recruitment in peripheral vs central cell attachment areas. g, h Quantification of average Rho activity sensor kinetics before, during and after Rac1 activation in A431 cells that co-express the Rho activity sensor, PA-Rac1 and control or Arhgef11/12 targeting siRNA oligonucleotides. g Measurement of average Rho activity sensor kinetics, corresponding to data shown in (h). h Quantification of the Rho activity response in the 25 s time frame during photoactivation. ****P < 0.0001; **P < 0.01; *P < 0.05; One-way ANOVA with Tukey’s (c, f) or Holm-Sidak’s (h) post test. Error bars represent standard error of the mean. Scale bars: 10 µm; 0.26 μm/pixel. All statistical tests were two-sided. Source data are provided as a Source Data file.