Fig. 2: Zeb1^∆M/Apoe^KO mice exhibit systemic inflammation and fat accumulation.

a Macrophage subpopulations were analyzed using published scRNAseq datasets of mouse atherosclerosis (GSE116240 and GSE149070)^3,4,11. b The share of macrophages out of CD45⁺ cells within the atherosclerotic plaque of Zeb1^WT/Apoe^KO (n = 4) and Zeb1^∆M/Apoe^KO (n = 5) mice was determined by FACS. c As in b, but the share of CD86^high macrophages was calculated out of the total number of macrophages (CD45⁺, CD11b⁺, F4/80⁺). (n = 3,4). d As in b, but the share of CD86^high macrophages was calculated out of total CD45⁺ cells. (n = 3, 4). e Stacked bar chart for (c) and (d). f–h As in c–e, but for CD9^high macrophages. i Total body weight of Zeb1^WT/Apoe^KO (n = 10) and Zeb1^∆M/Apoe^KO (n = 13) mice at the end of the feeding protocol. j The share of macrophages out of CD45⁺ cells infiltrating the WAT of Zeb1^WT/Apoe^KO and Zeb1^∆M/Apoe^KO mice. (n = 4). k and l Zeb1 and Il1b mRNA levels in the macrophages infiltrating the WAT of Zeb1^WT/Apoe^KO and Zeb1^∆M/Apoe^KO mice (n = 3). m Serum levels of IL1β (n = 13), IL2 (n = 13,12), IL12 (n = 12,14), and CCL2 (n = 12,13) from Zeb1^WT/Apoe^KO and Zeb1^∆M/Apoe^KO mice at the end of the feeding protocol. n Serum levels of leptin in Zeb1^WT/Apoe^KO (n = 8) and Zeb1^∆M/Apoe^KO (n = 10) mice. o Glucose tolerance test in Zeb1^WT/Apoe^KO (n = 7) and Zeb1^∆M/Apoe^KO (n = 6) mice at the end of the feeding protocol plus 16 h of fasting. p Liver weight of Zeb1^WT/Apoe^KO (n = 5) and Zeb1^∆M/Apoe^KO (n = 6) mice. q Representative images of liver sections from Zeb1^WT/Apoe^KO and Zeb1^∆M/Apoe^KO mice stained with H&E. Scale bar: 20 μm. r As in q, sections were stained for ORO. Representative images and quantification. Scale bar: 20 μm. (n = 6). s Lysates from the livers of Zeb1^WT/Apoe^KO and Zeb1^∆M/Apoe^KO at the end of the feeding protocol plus 12 h of fasting were blotted for mSREBP1c (clone 2A4, dilution 1/1000) and GAPDH (1E6D9, 1/20,000) as loading control. (n = 3). t Serum levels of total cholesterol (n = 11,13), free colesterol (n = 8,9), HDL (n = 7,9), and LDL (n = 11,13) in Zeb1^WT/Apoe^KO and Zeb1^∆M/Apoe^KO mice at the end of the feeding protocol. Graphs represent mean values ± SEM with two-tailed unpaired Student’s t test. p ≤ 0.001 (***), p ≤ 0.01 (**) or p ≤ 0.05 (*) levels, or non-significant (ns) for values of p > 0.05. The raw data, along with p values from statistical analyses are included in the Source Data file.