Fig. 1: Schematic illustration of ANCA method for the detection of target nucleic acid. | Nature Communications

Fig. 1: Schematic illustration of ANCA method for the detection of target nucleic acid.

From: ANCA: artificial nucleic acid circuit with argonaute protein for one-step isothermal detection of antibiotic-resistant bacteria

Fig. 1

First, Ago/G1 and Ago/G2 complexes hybridize and cleave target DNA, generating T1. Second, Ago/T1 complex forms, and it hybridizes and cleaves R, releasing Output and T2. Third, Ago/T2 complex forms, and it hybridizes and cleaves R*, generating T1 and thus completing ANCA. In the presence of target DNA, ANCA is repeatedly carried out and fluorescence signal increases. P stands for phosphate attached to the 5’ end.

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