Fig. 2: Evaluation of ANCA method. | Nature Communications

Fig. 2: Evaluation of ANCA method.

From: ANCA: artificial nucleic acid circuit with argonaute protein for one-step isothermal detection of antibiotic-resistant bacteria

Fig. 2

Schematic illustration of (a) ANCA and (b) ANCA without R*. ANCA method enables exponential amplification through positive feedback. ANCA without R* cleaves target DNA and R, but no further reaction transpires. c Time-dependent fluorescence intensities during ANCA reaction (left) and corresponding PAGE analysis result (right) under various components. d Time-dependent fluorescence intensities during ANCA reaction without R* (left) and corresponding PAGE analysis result (right) under various components. [Target DNA] = 1 nM, [R] = 500 nM, [R*] = 500 nM, [G1] = 25 nM, [G2] = 25 nM, [Ago] = 200 nM, [MgCl2] = 10 mM, [NaCl] = 75 mM, and [BSA] = 10 µg/mL. The experiments were independently replicated three times.

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