Fig. 9: Rv2067c gives survival advantage to Mtb. | Nature Communications

Fig. 9: Rv2067c gives survival advantage to Mtb.

From: The Mycobacterium tuberculosis methyltransferase Rv2067c manipulates host epigenetic programming to promote its own survival

Fig. 9

a Survival of intracellular bacilli post 24 and 48 h of infection in THP-1 with ΔRv2067c (filled triangle), WtMtb (open circle), ΔRv2067c:RXR (open triangle) and ΔRv2067c:comp (filled square). n = 3 independent infections. Three technical replicates were kept during each infection. b Bacillary burden in lungs of mice infected with ΔRv2067c (filled triangle), WtMtb (open circle) and ΔRv2067c:comp (filled square), 4- and 8-week p.i (n = 6 animals per group per time point, two technical replicates for plating were kept). a, b Data is plotted as mean and error bars represent SD. P-values depicted on the graphs were calculated using unpaired two-tailed Student’s t-test. c Gross pathology of the lungs of BALB/c mice infected with ΔRv2067c, WtMtb and ΔRv2067c:comp harvested 8 weeks after infection. d Haematoxylin and eosin–stained mouse lung sections 8 weeks p.i with ΔRv2067c, WtMtb and ΔRv2067c:comp.The pathology sections show granuloma (G), alveolar space (AS), Alveolar consolidation (AC) and bronchial lumen (B). All images were taken at 4X magnification and scale bars = 50 μm. e Graph depicts granuloma score of lung sections of mice infected with ΔRv2067c, WtMtb and ΔRv2067c:comp, 8 weeks p.i. (n = 5 animals). The error bars represent SD. Source data are provided as a Source Data file.

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