Fig. 7: Cell type-specific function of operant Dop1R2 activity in adult satiety.

a OptoPAD setup allowing light stimulation upon feeding action. Flies expressing optoXRs in a subset of MBONs (MBONγ5β’2a,β2mp and β2mp-bilateral) related to behavioral valence receive a light stimulus (1 s 525 nm 400 μW/cm²) every time they feed on the sucrose drop. b Cumulative sips over time for flies expressing optoDop1R2^V2 using MB011B-Gal4 without or with light stimulation (mean ± SEM, n = 58, 63 animals). c Total sips at 60 min for flies expressing optoDop1R2^V2 using MB011B-Gal4 without or with light stimulation (n = 58, 63 animals, two-tailed Mann-Whitney test). d Total sips at 60 min for flies expressing optoDop1R1^V2 using MB011B-Gal4 without or with light stimulation (n = 65, 65 animals, two-tailed Mann-Whitney test). e Total sips at 60 min for flies expressing Dop1R2^RNAi control or with MB011B-Gal4 (n = 54, 50 animals, two-tailed Mann-Whitney test). f Total sips at 60 min for flies expressing Dop1R1^RNAi control or with MB011B-Gal4 (n = 41, 47 animals, two-tailed Mann-Whitney test). All boxplots depict 75th (top), median (central line) and 25th (bottom) percentile, whiskers depict 99th (top) and 1st (bottom) percentile. All violin plots with single data points depict data distribution, dotted lines depict 75th (top) and 25th (bottom) percentile, solid central line the median. Source data and statistical details are provided as a Source Data file.