Table 1 Transgenic Drosophila lines used in this study

Dop1R1^KO-Gal4

Knockout-Gal4 of Dop1R1

BDSC# 84714

UAS-Dop1R1^RNAi

Knockdown of Dop1R1

BDSC# 62193

UAS-Dop1R2^RNAi

Knockdown of Dop1R2

BDSC# 51423

Dop1R2^KO-Gal4

Knockout-Gal4 of Dop1R2

BDSC# 84715

201y-Gal4

Expresses GAL4 in the mushroom body

BDSC# 64296

H24-Gal4

Expresses GAL4 in the mushroom body

BDSC# 51632

UAS-bPAC

Optogenetic cAMP induction

Stierl et al. (ref. ⁶¹),

BDSC# 78788

UAS-optoDop1R1^V2

Optogenetic Dop1R1 activation

This study

UAS-optoDop1R2^V2

Optogenetic Dop1R2 activation

This study

UAS-optoDop1R1^V1

Optogenetic Dop1R1 activation

This study

ppk-Gal4

Expresses GAL4 in C4da neurons

Han et al. (ref. ⁹⁹)

UAS-CsChrimson-GFP

Optogenetic activation

Klapoetke et al. (ref. ⁶²),

BDSC# 55136

UAS-Gflamp1

cAMP reporter

Wang et al. (ref. ⁶³)

UAS-Gcamp6s

calcium reporter

Chen et al. (ref. ⁶⁵)

MBONg1g2-Gal4

Expresses GAL4 in MBON-g1,g2

Saumweber et al. (ref. ⁵⁶)

Pdf-Gal4

Expresses GAL4 in I-LN_v and s-LN_v

BDSC# 6899

MB011B-Gal4

Expresses GAL4 in valence-encoding MBONs

Aso et al. (ref. ⁸⁴)

2U

w¹¹¹⁸ (isoCJ1) Canton-S derivative

Tully et al. (ref. ¹⁰⁰)

OK107-Gal4

Expresses GAL4 in the mushroom body

BDSC# 854

tub-Gal80^ts

Expresses temperature sensitive GAL80 in all cells

BDSC# 7019

R21B06-splitGal4^DBD

Expresses GAL4^DBD in the mushroom body

Aso et al. (ref. ⁵²)

6xCRE-splitGal4^AD

Expresses GAL4^AD in a Cre-dependent manner, VK27 insertion

This study, see Siegenthaler et al. (ref. ⁵⁹)

UAS-myr::tdTomato

Fluorescent reporter line

Pfeiffer et al. (ref. ¹⁰¹),

BDSC# 32223

UAS-Dop1R1GFP₁₁, UAS-spGFP_1-10

Dop1R1 knock-in line with C-terminal GFP₁₁ tag

Kondo et al. (ref. ⁵⁸)

10xUAS-myr::GFP

Fluorescent reporter line

Pfeiffer et al. (ref. ¹⁰¹),

BDSC# 32197