Fig. 3: Clustering analysis of differential gene expression patterns during myogenic differentiation of pgEpiSCs.
From: Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells
a PCA plot of three populations during the myogenesis of pgEpiSCs in vitro. Colors indicate different cell populations. b Ternary plot of three populations during the myogenesis of pgEpiSCs in vitro. Key markers of different cell populations are indicated with different colors. c Loading scores of the genes for PC1 during the myogenesis of pgEpiSCs in vitro. d A heatmap of clusters representative of each cell population during the myogenesis of pgEpiSCs in vitro shows similar expression patterns of genes in the same cluster. e Enriched gene ontology (GO) terms in representative clusters that had high q values in different cell populations during myogenic differentiation of pgEpiSCs. f, g Expression of genes related to myofibre maturation and collagen formation during myogenic differentiation of pgEpiSCs as evaluated by qPCR. WT undifferentiated pgEpiSCs, Myo-Dif terminally differentiated cells after N2 treatment. For (a, b, d), pgEpiSCs-MPCs: pgEpiSCs-derived myogenic progenitor cells; pgEpiSCs-MCs: mature muscle fiber cells after N2 treatment. For (f, g), error bars indicate means ± SD, n = 3. *** p < 0.001, **** p < 0.0001. Similar results were obtained in three independent experiments and represent significant using two-tailed student’s t test. Exact P values are listed in Source Data Fig. 3.
