Fig. 4: Metabolomic analysis of pgEpiSCs and pgEpiSCs-MCs.
From: Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells
a Graphic illustration of the workflow for acquiring metabolome data from pgEpiSCs undergoing myogenic differentiation. QC quality control, DMA Differential metabolites analysis. b PCA of pgEpiSCs and pgEpiSCs-MCs metabolome samples. c Volcano plot of differentially abundant metabolites between pgEpiSCs and pgEpiSCs-MCs. d Heatmap of differentially abundant metabolites in pgEpiSCs and pgEpiSCs-MCs. The color (blue to red) indicates metabolite abundance from low to high. e Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway terms are enriched among metabolites of myogenic differentiation between pgEpiSCs and pgEpiSCs-MCs. f RNA-Seq data showing the expression of genes involved in acetyl-CoA generation, ATP generation, and glycolysis in pgEpiSCs and pgEpiSCs-MCs. g Box plot of differentially abundant metabolites (succinic acid, glucose 6-phosphate) in pgEpiSCs and pgEpiSCs-MCs. The center line denotes the median, while the box contains the 25th to 75th percentiles and the whiskers mark 1.5x interquartile range. h qRT‒PCR analysis of mRNA levels of genes associated with acetyl-CoA production, ATP production, and glycolysis. i Total ATP levels in pgEpiSCs and pgEpiSCs-MCs. The ATP concentration was converted to μmol/mg of protein to eliminate errors due to differences in protein amounts in sample preparation. For (h, i), WT: undifferentiated pgEpiSCs; Myo-Dif: mature muscle fiber cells after N2 treatment. Error bars indicate means ± SD, n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001. Represent significant using two-tailed student’s t test and similar results were obtained in three independent experiments. Exact P values are listed in Source Data Fig. 4.
