Fig. 3: Astrocytic IR regulation of entrainment to feeding is sexually dimorphic in mice. | Nature Communications

Fig. 3: Astrocytic IR regulation of entrainment to feeding is sexually dimorphic in mice.

From: Astrocytic insulin receptor controls circadian behavior via dopamine signaling in a sexually dimorphic manner

Fig. 3

a Diagrams showing the food restriction paradigms. b Average waveforms of control and IRcKO mice under food restriction (RF) from ZT 4–10 (RF 6 h), ZT 4–8 (RF 4 h), and after the INS and glucose bolus administration. Purple areas in the graphs indicate the feeding time. Data are presented as mean ± s.e.m. (Males: n = 24–19 (RF6h), n = 29–24 (RF4h), n = 5 (phase shift) animals in control-mutant pairs. Females: n = 5–8 animals in control-mutant pairs). Two-way ANOVA. c Representative actograms depicting the circadian behavior of control and IRcKO mice ad libitum, during the RF 4 h and the phase shift induced by the administration of INS and glucose. Purple areas indicate the feeding time; the asterisk indicates the day mice received the bolus of INS and glucose (at ZT12); orange areas show the expected phase shift after the administration of INS and glucose. d Percentage of the hourly FAA in control and IRcKO mice. Data are presented as mean ± s.e.m. (Males: n = 24–20 (RF6h), n = 29–25 (RF4h), n = 5 (phase shift) animals in control-mutant pairs. Females: n = 5–8 animals in control-mutant pairs). Two-way ANOVA. Source data are provided as a Source Date file.

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