Table 1 Summary of TiLV polymerase structures
Structure No. | Short name | Resolution | RNA/promoter mode | Description | PDB/EMDB |
|---|---|---|---|---|---|
vRNA initiation | |||||
1 | Full vRNA initiation with CTP | 2.73 Å | vRNA/A | Full mode A structure with 3′ end in the active site and incoming CTP at +1 | PDB 8PSN EMD-17857 |
2 | Core vRNA initiation with CTP | 2.40 Å | vRNA/A | Core only mode A structure with 3′ end in the active site and incoming CTP at +1 | PDB 8PSO EMD-17858 |
cRNA pre-initiation | |||||
3 | Core cRNA pre-initiation mode A | 2.65 Å | cRNA/A | Core only mode A structure with 3′ end in the active site | PDB 8PSQ EMD-17860 |
4 | Core with endo cRNA pre-initiation mode A | 2.80 Å | cRNA/A | Core with endo, mode A structure with 3′ end in the active site | PDB 8PT7 EMD-17869 |
cRNA mode B | |||||
5 | Closed core with endo cRNA mode B | 2.83 Å | cRNA/B | Core with endo, mode B structure with 3′ end in the secondary site (weak duplex) | PDB 8PSS EMD-17861 |
vRNA pre-initiation | |||||
6 | Core vRNA pre-initiation mode A | 3.18 Å | vRNA/A | Core, mode A structure with 3′ end in the active site | PDB 8PSU EMD-17862 |
vRNA elongation | |||||
7 | Full vRNA elongation with CpNHpp | 2.96 Å | vRNA | Full elongation structure stalled with CpNHpp at +1 | PDB 8PSX EMD-17864 |
8 | Full vRNA elongation with CpNHpp and additional mode B promoter | 2.42 Å | vRNA/B | Full elongation structure stalled with CpNHpp at +1 with additional mode B promoter | PDB 8PSZ EMD-17865 |
vRNA mode B | |||||
9 | Full with open core vRNA mode B | 2.59 Å | vRNA/B | Full mode B structure with 3′ end in the secondary site (strong duplex) | PDB 8PT2 EMD-17866 |
10 | Open core with rotated endo-NLS vRNA mode B | 2.73 Å | vRNA/B | Core with rotated Endo-NLS mode B structure (open, strong duplex) | PDB 8PTH EMD-17871 |
11 | Closed core with rotated endo-NLS vRNA mode B | 2.86 Å | vRNA/B | Core with rotated Endo-NLS mode B structure (closed, weak duplex) | PDB 8PTJ EMD-17872 |
Replicase | |||||
12 | Replicase initiation with CpNHpp | 2.90 Å | vRNA/B | Core with rotated Endo-NLS and CBD (replicase conformation) mode A with 3′ end in the active site and incoming CpNHpp at +1 | PDB 8PT6 EMD-17868 |
Pre-termination | |||||
13 | Full vRNA pre-termination with GpCpp | 3.13 Å | vRNA | Full elongation structure stalled with GpCpp at +1 close to the template 5′ hook | PDB 8QZ8 EMD-18772 |
