Table 1 Binding rates and dissociation constants of S tail peptides interacting with coatomer WD40 domains1
From: A single C-terminal residue controls SARS-CoV-2 spike trafficking and incorporation into VLPs
Peptide sequence | WD40 analyte | Equilibrium KD (µM) | kon (103/Ms) | koff (10−1/s) |
|---|---|---|---|---|
GVKLHYT2 | α | 1.40 ± 0.15 | 152.13 ± 3.45 | 1.45 ± 0.01 |
GVKLHYT | β‘ | n.d. | n.d. | n.d. |
GVKLHYE2 | α | 0.31 ± 0.01 | 58.96 ± 0.54 | 0.24 ± 0.003 |
GVKLHYE | β‘ | 2.3 ± 0.23 | 2424.7 ± 178.3 | 12.7 ± 0.3 |
GVKLHAT | β‘ | n.d. | n.d. | n.d. |
KEVYLHG | β‘ | n.d. | n.d. | n.d. |
GVKLHYT | β‘-Tyr33Ala | n.d. | n.d. | n.d. |
GVKLHYE | β‘-Tyr33Ala | n.d. | n.d. | n.d. |
GVKLHYT (amidated) | α | n.d. | n.d. | n.d. |
GVKLHYE (amidated) | α | 2.3 ± 0.7 | 706.2 ± 56.3 | 8.8 ± 0.2 |
KEVYLHG | α-Tyr139Ala | n.d. | n.d. | n.d. |
GVKLHYT | α-Arg13Ala | 8.5 ± 2.0 | 147.7 ± 7.0 | 3.8 ± 0.07 |
GVKLHYE | α-Arg13Ala | 3.0 ± 0.2 | 132.5 ± 4.5 | 2.2 ± 0.08 |
GVKLHYT | α-Arg300Ala | 3.6 ± 0.4 | 360.1 ± 9.4 | 8.1 ± 0.07 |
GVKLHYE | α-Arg300Ala | 4.2 ± 0.4 | 136.6 ± 2.6 | 4.3 ± 0.03 |
GVKLHYT | α-Lys15Ala | n.d. | n.d. | n.d. |
GVKLHYE | α-Lys15Ala | n.d. | n.d. | n.d. |
GVKLHYE | β‘-Lys17Ala | n.d. | n.d. | n.d. |
CCKFDEDDSEPVLKGVKLHYT | α | 1.7 ± 0.083 | 99.3 ± 1.5 | 2.67 ± 0.01 |
CCKFDEDDSEPVLKGVKLHYE | α | 0.1 ± 0.005 | 59.9 ± 1.3 | 0.037 ± .002 |
CCKFDEDDSEPVLKGVKLHYT3 | α | 1.1 ± 0.1 | 145.5 ± 4.4 | 2.9 ± 0.03 |
CCKFDEDDSEPVLKGVKLHYE3 | α | 0.1 ± 0.002 | 60.8 ± 1.3 | 0.03 ± 0.002 |
