Fig. 2: mTOR inhibition triggers phosphorylation of FAM134B and FAM134C by CK2. | Nature Communications

Fig. 2: mTOR inhibition triggers phosphorylation of FAM134B and FAM134C by CK2.

From: The function of ER-phagy receptors is regulated through phosphorylation-dependent ubiquitination pathways

Fig. 2

Validation of kinase screening HITs counteracting the effect of Torin1 in FAM134B- (A) or FAM134C- (B) overexpressing cells. Left panels: analytic representation of FAM134B or FAM134C-induced ER-phagy changes upon treatment with a dilution series of selected compounds (SGC-CK2-1 (CK2i), VE-822 (ATRi), KU-60019/KU-55933 (ATMi), CHIR-124 (Chk1i) and MK-2206 2HCL (AKTi)) ranging from 0.01 to 4 µM combined with Torin1 (T) treatment. DMSO was set to represent 100% inhibition of FAM134B- and FAM134C-induced ER-phagy while Torin1 represents 0%. Averaged RFP/GFP ratio measured in response to HIT compound treatments was normalized at each time point to the averaged RFP/GFP ratio of DMSO and Torin1. The area under the highest concentration curve was colored in gray for better visualization. Right panels: growth of U2OS cells overexpressing FAM134B or FAM134C treated with indicated compounds and concentrations and recorded over a time period of 48 h (data from two time points are shown in the bar graphs). Data information: data shown in (A, B) represent averaged data obtained from n = 3 individual wells via the IncuCyte® S3, each view containing >100 cells. Data are mean ± SD, [au] arbitrary unit. Source data are provided as source data file.

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