Fig. 3: Mfsd2b-mediated S1P efflux controls glucose uptake by modulating intracellular S1P. | Nature Communications

Fig. 3: Mfsd2b-mediated S1P efflux controls glucose uptake by modulating intracellular S1P.

From: Sphingosine-1-phosphate suppresses GLUT activity through PP2A and counteracts hyperglycemia in diabetic red blood cells

Fig. 3

A Intracellular S1P levels and (B) corresponding S1P efflux in RBC from Mfsd2b+/+ and Mfsd2b–/– mice loaded or not with sphingosine and either S1P unloaded or not with BSA (n = 7 each). C Glucose uptake in RBC from Mfsd2b+/+ and Mfsd2b–/– treated as in (A) (n = 14 each). Inset depicts GLUT4 cell surface localization. D Intracellular S1P levels and (E) corresponding S1P efflux in HEK293-SphK1-wt and HEK293-SphK1-Mfsd2b cells treated with vehicle or 1 µM Sph followed by incubation without or with 1% BSA for 30 min (n = 4 each). F Glucose uptake rate in the same cells and treatments as in (E) (n = 4 each). Data are presented as mean ± sd and tested with stack matched two-way ANOVA (# for comparing Mfsd2b+/+ vs. Mfsd2b –/–; * for comparing treatments); in (E), there is a significance if a paired t test is used. ns= not significant; p# < 0.05; p## < 0.01; p* < 0.05; p** < 0.01; p*** < 0.001; p**** < 0.0001.

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