Fig. 4: FTY720 and FTY720-P transport into RBC occurs independently of Mfsd2b with FTY720-P but not FTY720 suppressing glucose uptake. | Nature Communications

Fig. 4: FTY720 and FTY720-P transport into RBC occurs independently of Mfsd2b with FTY720-P but not FTY720 suppressing glucose uptake.

From: Sphingosine-1-phosphate suppresses GLUT activity through PP2A and counteracts hyperglycemia in diabetic red blood cells

Fig. 4

A + D Intracellular FTY720 (black color) and FTY720-P (blue color) levels and corresponding FTY720 and FTY720-P efflux (B + E) after loading with 1 µM FTY720 (A/B) and 1 µM FTY720-P (D/E) followed by incubation without or with 1% BSA for 20 min (n = 4 each). C Glucose uptake of RBC treated with FTY720 and (F) FTY720-P, respectively, with and without addition of 1% BSA (n = 4 each). Vehicle was EtOH for FTY720 and DMSO (0.1%) for FTY720-P. G Efflux of FTY720-loaded RBC (n = 6 each) and (H) FTY720-P-loaded RBC to 1% BSA in RBC from Mfsd2b+/+ and Mfsd2b−/− mice (n = 6 each). The ‘n’ in the (A–F) refers to individual mice. Data are presented as mean ± sd and tested with stack matched one-way ANOVA followed by a Tukey’s multiple comparison test; ns = not significant; p* < 0.05; P** < 0.01; P*** < 0.001; P**** < 0.0001.

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