Fig. 6: Paride requires stress responses for infection of dormant hosts. | Nature Communications

Fig. 6: Paride requires stress responses for infection of dormant hosts.

From: Phage Paride can kill dormant, antibiotic-tolerant cells of Pseudomonas aeruginosa by direct lytic replication

Fig. 6

a, b Deep-dormant cultures of P. aeruginosa PAO1 Δpel Δpsl (wildtype) and its ΔrelA ΔspoT derivative both grown in M9Rich were treated with antibiotics or phages (MOI ≈ 0.01) and viable CFU/ml as well as free phages were recorded over time. c Growing cultures of P. aeruginosa were treated with 1 mM of DL-serine hydroxamate for 12 h and then challenged with antibiotics or phages (MOI ≈ 0.01). Viable CFU/ml as well as free phages were recorded over time (see Fig. S8e for a control experiment without SHX). Data points and error bars show the average of three independent experiments and their standard error of the mean. Limits of detection are 2 log10 CFU/mL for viable cells and 3.6 log10 PFU/mL for free phages. Source data are provided as a Source data file.

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