Fig. 5: Disruption of late NHEJ processes promotes MMEJ deletions and translocations. | Nature Communications

Fig. 5: Disruption of late NHEJ processes promotes MMEJ deletions and translocations.

From: iMUT-seq: high-resolution DSB-induced mutation profiling reveals prevalent homologous-recombination dependent mutagenesis

Fig. 5

A Metagene line plot of base substitution and deletion rates delta to -DSB and then delta to control siRNA, quantified by iMUT-seq. B Same as (A) but for XRCC4 siRNA. C Same as (A) but for LIG4 siRNA. D Average rate of microhomology-mediated end-joining (MMEJ) per DSB loci, points represent each biological replicate and error bars are S.D., all statistics are done relative to the control siRNA result using a paired, two-sided t-test, * p < 0.05, ** p < 0.01, *** p < 0.001, n = 6 independent biological replicates for CTRLsi and 3 for all other conditions. E Same as (D) but for translocations per DSB locus. F Heatmap of translocation quantified by iMUT-seq with XRCC4 depletion, each row and each column represent different iMUT-seq amplicons of either HR-prone or NHEJ prone loci with each cell being the translocation rate of the two loci, delta to control siRNA. G Same as (F) but for LIG4 siRNA. H Representative images of metaphase spreads with 2 Gy irradiation and with either control, XRCC4 or LIG4 siRNA, scalebar is 10 µm. I Quantification of chromosomal aberrations in the metaphase spreads from (H) as a percentage of total chromosomes, points represent each biological replicate, centre is mean of replicates, and error bars are S.D., statistics done relative to control siRNA using a paired, two-sided t-test, ** p < 0.01, *** p < 0.001, n = 3 independent biological replicates. Source data and statistics are provided with this paper.

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