Fig. 1: Increased SIRT6 expression is positively related to asthma severity.

a Schematic illustrating an established HDM/LPS-induced acute severe asthma (ASA) mouse model. b, c qRT-PCR analysis of the expression of the SIRT family (left margin) in mouse lung tissues. Results were normalized to those of the gene encoding β-actin. d, e Western blot analysis of the SIRT family (SIRT1, SIRT3, SIRT5, and SIRT6). Quantification of SIRT6 expression was analyzed by using Image J software. f, g mRNA levels of Sirt6 in peripheral blood from healthy participants (n = 11) and asthmatic patients (n = 33). h, i Asthmatic patients were divided into mild to moderate asthma (n = 16) and severe asthma (n = 17) according to their disease severity. mRNA and protein levels of Sirt6 in peripheral blood were assessed. j, k Postbronchodilator forced expiratory volume in 1 second (FEV₁) was assessed. Correlation between Sirt6 expression in peripheral blood and FEV₁ was determined by Spearman analysis. l, m Asthma control test (ACT) score was analyzed. Correlation between Sirt6 expression in peripheral blood and ACT score was determined by Spearman analysis. n, o Percentage of neutrophils in peripheral blood was analyzed. Correlation between Sirt6 expression and neutrophils percentage in peripheral blood was determined by Spearman analysis. Data are shown as means ± SEM and three or more independent experiments were performed. Significance was calculated by Two-tailed unpaired Student’s t-test for (a–e–f, g–l); one-way ANOVA followed by Tukey’s post-hoc test for (h–j–n).