Fig. 3: AEP-derived organoids elucidate dynamics of alveolar epithelial differentiation. | Nature Communications

Fig. 3: AEP-derived organoids elucidate dynamics of alveolar epithelial differentiation.

From: Alveolar epithelial progenitor cells require Nkx2-1 to maintain progenitor-specific epigenomic state during lung homeostasis and regeneration

Fig. 3

A scVelo RNA velocity UMAP showing differentiation dynamics (A) and pseudotime inferred from RNA velocity (A”) in AEP organoids. B Slingshot trajectory analysis and pseudotime inference of AEP organoids demonstrate similar lineage relationships to RNA velocity. C–E Lineage drivers defined by CellRank for differentiation of pAEP/AEP to AT2 cells (C), AT1 cells via AT1tr (D), and Krt8 cells (E). F Heatmap showing major cell markers differentiating cell states in the alveolar epithelium. G Model of cellular relationships and AT1 differentiation inferred from alveolar organoids.

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