Fig. 3: Changes in IgM N-glycosylation correlate with PBMC glycosyltransferase/glycosidase mRNA expression.
From: IgM N-glycosylation correlates with COVID-19 severity and rate of complement deposition
a COVID-19 trajectory 1–3 (nonsevere, n = 12 biologically independent samples) and trajectory 4 and 5 (severe, n = 10 biologically independent samples) expression of glycosyltransferases were significantly different between MAN1A2 (p = 0.007), MAN2A1 (p = 0.025), ST3GAL4 (p = 0.018), and ST6GALNAC2 (p = 0.025). Data are presented as mean values +/– S.D. The role of each glycosidase and glycosyltransferase are depicted below. b Total mannose on IgM positively correlated with MAN1A2 and TMTC3 expression while negatively correlating with ST3GAL4 expression. The summation of sialic acids on IgM positively correlated with ST3GAL4 expression. mRNA expression is graphed as mean +/– S.D. Statistical significance was determined using a two-sided Kruskal-Wallis test with *p < 0.05 and **p < 0.01. Associations between IgM N-glycosylation and mRNA expression were determined using simple linear regression analysis. Source data are provided as a Source Data file.
